Themechanismofelectro-acupunctureatShuandMuacupointsoflungandlargeintestinemeridiansforrelievingacutegastrointestinalinjuryinratswithacutepancreatitisZhen-YanLiu,JunWu,Si-ChengLi,HangSu,LingYuan,HuiGuo,Mei-HuaWan,Wen-FuTang

Highlights：Basedonthetheoryofexterior-interiorrelationshipbetweenthelungandthelargeintestine,themechanismofelectro-acupunctureatShuandMuacuacupointsforrelievinggastrointestinalinjuryofAPratswasexplored.Wefoundthatelectro-acupunctureatshuandmuacupointsoflungandlargeintestinemeridians(BL13,BL25,LU1,ST25)canrelieveAPratsgastrointestinalinjurybyrebalancinginflammatoryandanti-inflammatorycytokines,andmakinghigherlevelofDAO,VIP,CCK.

文章通过采用牛黄胆酸钠逆行胰胆管微量泵泵入法造模后，探究了电针针刺大鼠肺与大肠经俞募穴减轻急性胰腺炎胃肠损伤的机理。结果表明，电针针刺肺经与大肠经俞穴及募穴治疗AP改善胃肠损伤，与其重建促炎和抗炎细胞因子的平衡及提高DAO、VIP、CCK水平等相关。

Abstract:Objectives:Thisstudyaimstoexplorethemechanismofalleviatinggastrointestinalinjuryofratswithacutepancreatitis(AP)byelectro-acupunctureatShuandMuacupointsoflungandlargeintestinemeridians.Methods:Allratswererandomlydividedinto5groups(n=40):groupA(DaChengqiDecoction(DCQD),n=8),groupB(BackShuacupoints(BL13，BL25)andDCQD,n=8),groupC(FrontMuacupoints(LU1，ST25)andDCQD,n=8),groupD(FrontMuandBackShuacupoints(BL13，BL25，LU1，ST25)andDCQD,n=8),andgroupE(theratmodelofAP,n=8).TheratmodelofAPwasestablishedbyretrogradeinfusionof3%sodiumtaurocholateintobiliarypancreaticduct.ExceptinggroupE,allothergroupswereofferedwithDCQDbyintragastricinfusionwithin24hafterestablishingmodels.Inaddition,groupB,CandDreceived3timeselectro-acupunctureand40minseachtimewithin24hoursafterestablishingmodels,theheartblood,pancreatictissueandintestinaltissueweretook12hafterintragastricinfusiontotestthelevelsoftumornecrosisfactor(TNF-α),interleukin4(IL-4),diamineoxidase(DAO),cholecystokinin(CCK),andvasoactiveintestinalplypeptide(VIP).Thepancreatictissueandintestinetissueweretakenforpathologyobservation.Results:ComparedwithgroupA,thelevelofTNF-αwaslowerandthelevelofIL-4washigheringroupB(P0.05).GroupB,CandDhadhigherCCKandDAOlevelsthangroupA(P0.05).GroupChadthehighestCCKlevelofthethreegroups(P0.05)andthelevelofDAOwasbetteringroupB,D(P0.05).GroupB,DhadhigherVIPlevelthangroupAandgroupBwasthebestofthem(P0.05).Conclusions:Themechanismofelectro-acupunctureatShuandMuacuacupointsforrelievinggastrointestinalinjuryofAPratsisassociatedwiththerebalancebetweeninflammatoryandanti-inflammatarycytokines,andtheincreasingofDAO,VIPandCCKlevel.

摘要目的：探究基于电针针刺大鼠肺与大肠经俞募穴减轻急性胰腺炎胃肠损伤的机理。方法：将40只大鼠随机分为大承气汤组（大承气汤灌胃）；俞穴针药组（电针肺与大肠俞穴+大承气汤灌胃）；募穴针药组（电针肺与大肠募穴+大承气汤灌胃）；俞募针药组（电针肺与大肠俞募穴+大承气汤灌胃）和模型组,每组8只。所有大鼠均采用牛黄胆酸钠逆行胰胆管微量泵泵入法造模。俞穴针药组、募穴针药组、俞募针药组造模后24h内共针刺3次，留针时间为40min。大承气汤组及模型组造模后24h内共3次将大鼠固定于特制固定器上，40min/次。除模型组外所有大鼠均在模型建立后24h给药。给药后12h，取大鼠心血与胰、肠等组织，检测血液中的肿瘤坏死因子（TNF-α）、白细胞介素4(IL-4)、二胺氧化酶（DAO）、胆囊收缩素（CCK）、血管活性肠肽（VIP）。结果：募穴针药组与大承气汤组相比，TNF-α降低（P0.05），IL-4浓度升高（P0.05）。针药结合组较大承气汤组CCK、DAO水平提高，其中募穴针药组CCK提高较为明显（P0.05），俞穴针药组及俞募针药组DAO水平改善明显（P0.05）。俞穴针药与俞募针药组比大承气汤组VIP水平高，俞募针药组较募穴针药组提高VIP水平明显（P0.05）。结论：电针针刺肺经与大肠经俞穴及募穴治疗AP改善胃肠损伤，与其重建促炎和抗炎细胞因子的平衡及提高DAO、VIP、CCK水平等相关。

作者简介

刘贞艳，女，成都中医药大学针灸推拿学院七年制中医学（针灸英语方向）在读生，已发表学术论文两篇。

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